... cryopreservation leucocytes peripheral blood mononuclear cells polymorphonuclear cells inflammation • Freezing primary leucocytes in 50% complete RPMI, 40% autologous plasma and 10% DMSO did not alter cellular bioenergetics when maintained in LN 2 for up to 4 weeks. • Long-term cryopreservation...

.... Human peripheral blood mononuclear cells (PBMC) cultured in vitro with soluble RANKL and human macrophage colony-stimulating factor form osteoclasts. However, PBMC are heterogeneous, consisting of subsets of monocytes and lymphocytes as well as other blood cells. As the CD14 marker is strongly expressed...