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Keyword: radioimmunoassay
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Articles
Clin Sci (Lond) (2003) 104 (3): 303-312.
Published: 17 February 2003
... during a 2-h period by radioimmunoassay and analysed for pulsatile behaviour by Fourier transformation. All patients and controls had significant rhythmic oscillations in plasma ANP levels, and 11 patients with CHF and 10 controls had significant rhythmic oscillations in plasma BNP levels. The amplitude...
Articles
Clin Sci (Lond) (2002) 103 (2): 171-177.
Published: 19 July 2002
... radioimmunoassay. mRNA was measured with a semi-quantitative reverse transcriptase–PCR method using specific CCK and ribosomal protein L19 (RPL19) primers. CCK tissue concentration and CCK mRNA were significantly reduced in patients with atrophic mucosa [12.2 (range 6.9–17.5) pmol/g; CCK/RPL19 ratio 0.64 (0.30...
Articles
Clin Sci (Lond) (1999) 97 (2): 201-206.
Published: 29 June 1999
...: Professor E. B. Pedersen. 17 3 1998 19 4 1999 7 5 1999 The Biochemical Society and the Medical Research Society © 1999 1999 atrial natriuretic peptide brain natriuretic peptide Fourier transformation pulsatile secretion radioimmunoassay 201Clinical Science (1999) 97, 201...
Articles
Clin Sci (Lond) (1997) 92 (1): 59-62.
Published: 01 January 1997
... as express the receptors. Adrenomedullin induces vasorelaxation by activating adenylate cyclase and also by stimulating the release of nitric oxide. 2. We have developed a specific radioimmunoassay and measured the immunoreactivity of human adrenomedullin in the plasma of 58 male subjects: eight with...
Articles
Clin Sci (Lond) (1996) 90 (1): 37-46.
Published: 01 January 1996
... endothelin high-performance liquid chromatography human endocardial endothelial cells immunocytochemistry reverse transcriptase—polymerase chain reaction radioimmunoassay Clinical Science (1996) 90, 3 7 4 (Printed in Great Britain) 37 Expression of endothelin peptides and mRNA in the human heart C...
Articles
Clin Sci (Lond) (1995) 89 (4): 375-381.
Published: 01 October 1995
... radioimmunoassay. The effect of cholecystokinin-8 infusion on subsequent food intake in a standard test meal was compared with the effect of saline infusion in the same subjects. 3. Food intake (mean ± SEM) was significantly less during cholecystokinin (5092 ± 665 kJ) than during saline infusion (6418 ± 723 kJ, P...
Articles
Clin Sci (Lond) (1994) 87 (3): 319-322.
Published: 01 September 1994
... diseases have not yet been studied. 2. Plasma immunoreactive C-type natriuretic peptide concentrations were studied by radioimmunoassay in normal subjects, patients with congestive heart failure, non-dialysed patients with chronic renal failure and haemodialysis patients with chronic renal failure. The C...
Articles
Clin Sci (Lond) (1994) 86 (2): 149-158.
Published: 01 February 1994
... cerebrospinal fluid dopamine β-hydroxylase, we applied a homologous human dopamine β-hydroxylase radioimmunoassay to cerebrospinal fluid, in order to characterize the properties and stability of cerebrospinal fluid dopamine β-hydroxylase, as well as its relationship to central noradrenergic neuronal activity...
Articles
Clin Sci (Lond) (1993) 84 (1): 47-50.
Published: 01 January 1993
... in blood pressure in some haemodialysis patients. 17 6 1992 31 7 1992 © 1993 The Biochemical Society and the Medical Research Society 1993 anaemia blood pressure endothelin erythropoietin haemodialysis radioimmunoassay Clinical Science (1993) 84, 47-50 (Printed in Great...
Articles
Clin Sci (Lond) (1992) 83 (2): 205-211.
Published: 01 August 1992
... Society 1992 chromatography ganglioneuroblastoma neuroblastoma neuropeptide Y phaeochromocytoma plasma radioimmunoassay Clinical Science (1992) 83, 205-21 I (Printed in Great Britain) 205 Neuropeptide Y as a plasma marker for phaeochromocytoma, ganglionauroblastoma and neuroblastoma...
Articles
Clin Sci (Lond) (1990) 78 (3): 261-264.
Published: 01 March 1990
...Anthony P. Davenport; Michael J. Ashby; Patricia Easton; Sarah Ella; Joy Bedford; Claire Dickerson; Derek J. Nunez; Stephen J. Capper; Morris J. Brown 1. A radioimmunoassay has been developed for measuring endothelin-like immunoreactivity in human plasma using an antibody raised against endothelin...
Articles
Clin Sci (Lond) (1987) 73 (3): 277-283.
Published: 01 September 1987
... with control subjects. 17 12 1986 13 3 1987 © 1987 The Biochemical Society and the Medical Research Society 1987 alcohol withdrawal blood pressure prostacyclin prostaglandin E radioimmunoassay thromboxane A 2 urine Clinical Science (1987) 73, 277-283 277 Decreased...
Articles
Clin Sci (Lond) (1986) 71 (3): 291-297.
Published: 01 September 1986
...M. E. J. Lean; W. P. T. James; G. Jennings; P. Trayhurn 1. A solid-phase radioimmunoassay is described for the estimation of the uncoupling protein content of human brown adipose tissue mitochondria, as an index of thermogenic capacity. 2. The concentration of inner mitochondrial membrane...
Articles
Clin Sci (Lond) (1983) 64 (6): 655-659.
Published: 01 June 1983
...P. J. Ciclitira; E. S. Lennox 1. A rapid, sensitive specific radioimmunoassay for α- and β-gliadin has been developed using an antiserum raised in rabbits to A-gliadin, a component of α-gliadin. 2. The antigen used in the assay was α-gliadin labelled with 125 I; antigen-antibody complexes were...
Articles
Clin Sci (Lond) (1982) 62 (4): 355-360.
Published: 01 April 1982
... angiotensin I, angiotensin II or synthetic hog tetradecapeptide renin substrate. However, rat des-angiotensin I—angiotensinogen cross-reacted 100% with the angiotensinogen antibody. 2. A direct radioimmunoassay for rat angiotensinogen in plasma was developed and this enabled 5 fmol of this protein to be...
Articles
Clin Sci (Lond) (1982) 62 (4): 427-429.
Published: 01 April 1982
... measured by radioimmunoassay in both groups from samples taken before, and at regular intervals up to 48 h after, the oral dose. 2. The plasma half-time for the disappearance of the administered 1,25-(OH) 2 D 3 was estimated by determining the time for a 50% reduction from the peak increment of the sterol...
Articles
Clin Sci (Lond) (1981) 61 (3): 295-298.
Published: 01 September 1981
... sodium concentration observed in young SHRSP rats. 9 3 1981 23 3 1981 © 1981 The Biochemical Society and the Medical Research Society 1981 arginine vasopressin radioimmunoassay spontaneously hypertensive rats Clinical Science (198 1) 61,295-298 295 Vasopressin in the plasma of...
Articles
Clin Sci (Lond) (1980) 59 (4): 257-263.
Published: 01 October 1980
...L. J. Fraher; T. L. Clemens; S. E. Papapoulos; J. Redel; J. L. H. O'Riordan 1. A radioimmunoassay for 25,26-dihydroxycholecalciferol has been developed with an anti-serum raised in a sheep, tritiated 1,25-dihydroxycholecalciferol as tracer and synthetic 25,26-dihydroxycholecalciferol as standard...
Articles
Clin Sci (Lond) (1980) 58 (2): 139-144.
Published: 01 February 1980
... 1979 6 9 1979 © 1980 The Biochemical Society and the Medical Research Society 1980 antidiuretic hormone clearance arginine vasopressin kidney radioimmunoassay renal micropuncture Clinical Science (1980) 58, 139-144 Fate of vasopressin perfused into nephrons of Wistar and...
Articles
Clin Sci (Lond) (1979) 56 (5): 419-426.
Published: 01 May 1979
...N. M. Bass; R. E. Kirsch; S. A. Tuff; J. A. Campbell; J. S. Saunders 1. A radioimmunoassay was used to establish normal urine excretion of ligandin, a renal tubular protein, in rats, and to study the pattern of ligandin excretion after nephrotoxin administration. 2. Validation of radioimmunoassay...
Articles
Clin Sci Mol Med (1978) 54 (3): 329-332.
Published: 01 March 1978
... for 1α,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1α-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1α,25-[23,24- 3 H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of...
Articles
Clin Sci Mol Med (1978) 54 (2): 147-151.
Published: 01 February 1978
... 1977 © 1978 The Biochemical Society and the Medical Research Society 1978 angiotensin II cerebrospinal fluid polyacrylamide gel electrophoresis radioimmunoassay Clinical Science and Molecular Medicine (1978) 54, 147-151 Characterization of immunoreactive angiotensin in canine...
Articles
Clin Sci Mol Med (1978) 54 (1): 61-68.
Published: 01 January 1978
... Society and the Medical Research Society 1978 metabolism pancreas radioimmunoassay secretin Clinical Science and Molecular Medicine (1978) 54,61-68 Elimination of porcine secretin in pigs J. FAHRENKRUG, O. B. SCHAFFALITZKY DE MUCKADELL AND J. J. HOLST Department of Clinical Chemistry...
Articles
Clin Sci Mol Med (1978) 55 (s4): 125s-128s.
Published: 01 January 1978
... proteolysis. The 70 000 form was activated without change in molecular weight. 3. In addition to its enzymic activity, renin was measured by a direct radioimmunoassay, which revealed that the current acid treatment of plasma did not activate all the renin present. 4. Renin is stored as fully active 40 000...
Articles
Clin Sci Mol Med (1977) 52 (3): 329-332.
Published: 01 March 1977
... Research Society 1977 calcitonin Paget's disease of bone parathyroid hormone radioimmunoassay Clinical Science and Molecular Medicine (1977) 52, 329-332. SHORT COMM UNICA TION Plasma calcitonin in Paget's disease of bone J. A. KANIS G. HEYNEN AND R. J. WALTON") ("Nufleld Orthopaedic Centre...
Articles
Clin Sci Mol Med (1977) 52 (2): 183-191.
Published: 01 February 1977
...P. J. G. Avis 1. A quantitative radioimmunoassay was developed for the measurement of nanogram amounts of Tamm—Horsfall (TH) glycoprotein in the presence of serum proteins at concentrations above 30 mg/ml. 2. Specific anti-(TH-glycoprotein) antibodies were labelled with 125 I and these were usable...
Articles
Clin Sci Mol Med (1976) 51 (6): 525-536.
Published: 01 December 1976
...T. H. Thomas; M. R. Lee 1. Rabbit antisera against arginine-vasopressin (AVP) were evaluated for sensitivity and specificity in a radioimmunoassay based on the extraction of AVP from plasma and urine on to Florisil. 2. Comparison of the immunoreactivity of AVP with analogues showed that one...
Articles
Clin Sci Mol Med (1976) 51 (2): 111-125.
Published: 01 August 1976
...F. A. O. Mendelsohn 1. A method for extraction, partial purification and radioimmunoassay of angiotensin II in tissues and application of the method to the kidneys of sodium-deficient rats are described. 2. Angiotensin in acid—ethanol extracts of kidney were adsorbed on to a cation-exchange resin...
Articles
Clin Sci Mol Med (1976) 50 (6): 487-491.
Published: 01 June 1976
... simultaneous injection of insulin (44 nmol; 6 units). 14 1 1976 © 1976 The Biochemical Society and the Medical Research Society 1976 cyclic AMP cyclic GMP glucagon insulin radioimmunoassay Clinical Science and Molecular Medicine (1976) 50, 481-491. The effect of insulin on adenosine 3...
Articles
Clin Sci Mol Med (1976) 51 (s3): 299s-301s.
Published: 01 January 1976
...Julia H. Hauger-Klevene; P. Vecsei 1. The free deoxycorticosterone pool in the rat was measured by means of a radioimmunoassay in vivo. 2. The free deoxycorticosterone pool in rats with adrenal regeneration with normal or high blood pressure was similar. 3. In rats with adrenal regeneration, the...
Articles
Clin Sci Mol Med (1976) 51 (s3): 391s-394s.
Published: 01 January 1976
... Research Society 1976 angiotensin II cerebrospinal fluid diabetes insipidus rats extrarenal angiotensinogenase polyacrylamide gel electrophoresis radioimmunoassay Clinical Science and Molecular Medicine (1976) 51, 391s-394s. Effect of intraventricular perfusion of angiotensin I1 in conscious...
Articles
Clin Sci Mol Med (1976) 51 (s3): 399s-402s.
Published: 01 January 1976
... electrophoresis radioimmunoassay Clinical Science and Molecular Medicine ( I 976) 51, 3999-402s. Impermeability of the bloodxerebrospinal fluid barrier for angiotensin I1 in rats P. S C H E L L I N G , J . S. H U T C H I N S O N , U. G A N T E N , G . S P O N E R AND D. G A N T E N Department of Pharmacology...
Articles
Clin Sci Mol Med (1975) 48 (2): 97-105.
Published: 01 February 1975
...C. L. Cope; S. Loizou 1. Radioimmunoassay has been used to detect and estimate the urinary excretion of deoxycorticosterone (DOC) in normal, hypertensive and hypokalaemic subjects. The range of excretions in ten healthy normal subjects was 41–232 pmol (13.7–76.7 ng) daily, with a mean of 124 pmol...
Articles
Clin Sci Mol Med (1974) 48 (s2): 135s-159s.
Published: 01 January 1974
...D. R. Bangham; Iris Robertson; J. I. S. Robertson; C. J. Robinson; M. Tree © 1974 The Biochemical Society and the Medical Research Society 1974 angiotensin I angiotensin II bioassay radioimmunoassay renin renin-substrate Clinical Science and Molecular Medicine (1975) 48, 135s-I 59s...
Articles
Clin Sci Mol Med (1973) 45 (5): 607-623.
Published: 01 November 1973
...T. P. Jowett; J. D. H. Slater; R. D. Piyasena; R. P. Ekins 1. A rapid, robust, accurate and sensitive radioimmunoassay procedure is described for the measurement of plasma and urinary aldosterone. 2. A new, simple method is validated for the separation of free from bound moieties which has general...
Articles
Clin Sci Mol Med (1973) 45 (3): 411-415.
Published: 01 September 1973
.... Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. 3. Its rapidity and relative inexpensiveness makes radioimmunoassay at present the most suitable technique for large-scale population screening. 31 10 1972 12 6 1973 © 1973 The...
Articles
Clin Sci Mol Med (1973) 44 (2): 163-179.
Published: 01 February 1973
...Anne M. S. Grant; A. Neuberger 1. A specific and quantitative radioimmunoassay was developed for the measurement of low concentrations of human and rabbit Tamm—Horsfall glycoprotein in the presence of other proteins. Antibody-coated tubes were used as a solid phase in the assay and the optimum...
Articles
Clin Sci Mol Med (1973) 44 (1): 43-54.
Published: 01 January 1973
...S. Fukuchi; T. Takeuchi; T. Torikai 1. A simple, rapid radioimmunoassay of angiotensin I has been applied to the measurement of plasma renin activity. 2. Antibody to angiotensin I was raised in rabbits by injecting angiotensin I conjugated with rabbit serum albumin. 3. Angiotensin I was generated...
Articles
Clin Sci (1972) 43 (2): 209-218.
Published: 01 August 1972
.... Because the antibody used in the radioimmunoassay cross-reacted with larger angiotensin breakdown products and because these fragments are present in higher concentrations in venous than arterial plasma, samples of both were assayed. Measurements on venous plasma are referred to as immunoreactive material...