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Keywords: stable isotopes
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Articles

Measuring muscle protein synthesis in humans and the influence of nutritional state

Philip C. Calder, Nicolaas E.P. Deutz
Journal: Clinical Science
Clin Sci (Lond) (2022) 136 (19): 1425–1431.
https://doi.org/10.1042/CS20211171
Published: 14 October 2022
...Philip C. Calder; Nicolaas E.P. Deutz In 1982 and 2011, Clinical Science published papers that used infusion of stable isotope-labeled amino acids to assess skeletal muscle protein synthesis in the fasted and fed state and before and after a period of increased intake of omega-3 fatty acids...
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Articles

Substrate utilization in non-obese Type II diabetic patients at rest and during exercise

L.B. BORGHOUTS, A.J.M. WAGENMAKERS, P.L.L. GOYENS, H.A. KEIZER
Journal: Clinical Science
Clin Sci (Lond) (2002) 103 (6): 559–566.
https://doi.org/10.1042/cs1030559
Published: 30 October 2002
... oxidation is impaired during exercise in non-obese Type II diabetic patients. Stable isotope tracers of palmitate and glucose were infused for 2h at rest and 1h of bicycle exercise at 40% peak oxygen consumption ( V O 2 max) in volunteers with Type II diabetes and a healthy control group. At rest, plasma...
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Articles

Lymphocyte protein synthesis is increased with the progression of HIV-associated disease to AIDS

Giuseppe CASO, Peter J. GARLICK, Marie C. GELATO, Margaret A. MCNURLAN
Journal: Clinical Science
Clin Sci (Lond) (2001) 101 (6): 583–589.
https://doi.org/10.1042/cs1010583
Published: 26 October 2001
... 6 2001 13 6 2001 The Biochemical Society and the Medical Research Society © 2001 2001 AIDS HIV infection lymphocyte protein synthesis l-[2H5]phenylalanine stable isotopes 583Clinical Science (2001) 101, 583 589 (Printed in Great Britain) Lymphocyte protein synthesis...
View article titled, Lymphocyte protein synthesis is increased with the progression of HIV-associated disease to AIDS
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Articles

Effects of smoking and abstention from smoking on fibrinogen synthesis in humans

Kirsty A. HUNTER, Peter J. GARLICK, Iain BROOM, Susan E. ANDERSON, Margaret A. McNURLAN
Journal: Clinical Science
Clin Sci (Lond) (2001) 100 (4): 459–465.
https://doi.org/10.1042/cs1000459
Published: 20 March 2001
... concentration. albumin synthesis cardiovascular disease cigarette smoking fibrinogen synthesis stable isotopes Correspondence : Dr Margaret McNurlan (e-mail mcnurlan@surg.som.sunysb.edu ). 14 8 2000 11 12 2000 17 1 2001 The Biochemical Society and the Medical Research...
View article titled, Effects of smoking and abstention from smoking on fibrinogen synthesis in humans
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Articles

Small-intestinal mucosal protein synthesis and whole-body protein turnover in alcoholic liver disease

I. M. NAKSHABENDI, S. DOWNIE, R. I. RUSSELL, M. J. RENNIE
Journal: Clinical Science
Clin Sci (Lond) (1999) 97 (6): 633–638.
https://doi.org/10.1042/cs0970633
Published: 29 October 1999
...I. M. NAKSHABENDI; S. DOWNIE; R. I. RUSSELL; M. J. RENNIE We used stable-isotope-labelled amino acids to measure the effects of alcoholic liver disease (ALD) on whole-body protein turnover and small-intestinal mucosal protein synthesis. Groups comprising eight patients with ALD and eight healthy...
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Articles

Metabolism of lactose-[13C]ureide and lactose-[15N,15N]ureide in normal adults consuming a diet marginally adequate in protein

A. A. JACKSON, R. BUNDY, A. HOUNSLOW, J. L. MURPHY, S. A. WOOTTON
Journal: Clinical Science
Clin Sci (Lond) (1999) 97 (5): 547–555.
https://doi.org/10.1042/cs0970547
Published: 28 September 1999
... microflora in normal individuals. Correspondence : Professor Alan A. Jackson (e-mail iohn@soton.ac.uk ). 5 3 1999 4 6 1999 22 7 1999 The Biochemical Society and the Medical Research Society © 1999 1999 amino acids colon nitrogen protein stable isotopes urea...
View article titled, Metabolism of lactose-[13C]ureide and lactose-[15N,15N]ureide in normal adults consuming a diet marginally adequate in protein
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Articles

Variation in the apparent sensitivity of the insulin-mediated inhibition of proteolysis to amino acid supply determines the efficiency of protein utilization

Amelia FEREDAY, Neil R. GIBSON, Malcolm COX, Paul J. PACY, D. Joe MILLWARD
Journal: Clinical Science
Clin Sci (Lond) (1998) 95 (6): 725–733.
https://doi.org/10.1042/cs0950725
Published: 01 December 1998
... utilization, stable isotopes. Abbreviations : HP, high protein ; LP, low protein ; KIC, a-ketoisocaproic acid ; PPU, postprandial protein utilization. Correspondence: Professor Joe Millward. mediate the deposition. This regulatory response, mainly to the protein content of the meal, has been defined...
View article titled, Variation in the apparent sensitivity of the insulin-mediated inhibition of proteolysis to amino acid supply determines the efficiency of protein utilization
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Articles

Absence of glutamine isotopic steady state: implications for the assessment of whole-body glutamine production rate

Bernadette A. C. VAN ACKER, Karel W. E. HULSEWÉ, Anton J. M. WAGENMAKERS, Nicolaas E. P. DEUTZ, Bernard K. VAN KREEL, Dave HALLIDAY, Dwight E. MATTHEWS, Peter B. SOETERS, Maarten F. VON MEYENFELDT
Journal: Clinical Science
Clin Sci (Lond) (1998) 95 (3): 339–346.
https://doi.org/10.1042/cs0950339
Published: 01 September 1998
... Acker. 28 1 1998 9 4 1998 28 4 1998 The Biochemical Society and the Medical Research Society © 1998 1998 glutamine kinetics muscle stable isotopes 339Clinical Science (1998) 95, 339 346 (Printed in Great Britain) Absence of glutamine isotopic steady state: implications...
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Articles

Site of Insulin Resistance after Surgery: The Contribution of Hypocaloric Nutrition and Bed Rest

Jonas Nygren, Anders Thorell, Suad Efendic, K. Sree Nair, Olle Ljungqvist
Journal: Clinical Science
Clin Sci (Lond) (1997) 93 (2): 137–146.
https://doi.org/10.1042/cs0930137
Published: 01 August 1997
... on endogenous glucose production. 7 3 1997 27 3 1997 © 1997 The Biochemical Society and the Medical Research Society 1997 fasting glucose clamp technique glucose metabolism insulin resistance stable isotopes Clinical Science (1997) 93, 137-146 (Printed in Great Britain) Site...
View article titled, Site of Insulin Resistance after Surgery: The Contribution of Hypocaloric Nutrition and Bed Rest
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Articles

Determination of Urea Kinetics by Isotope Dilution with [ 13 C]Urea and Gas Chromatography–isotope Ratio Mass Spectrometry (GC–IRMS) Analysis

W. D. Kloppenburg, B. G. Wolthers, F. Stellaard, H. Elzinga, T. Tepper, P. E. De Jong, R. M. Huisman
Journal: Clinical Science
Clin Sci (Lond) (1997) 93 (1): 73–80.
https://doi.org/10.1042/cs0930073
Published: 01 July 1997
... have described a urea dilution method using the triple labelled [l3Cl5N2] urea isotope and gas chromatography-MS (GC-MS) analysis for study- Keywords: gas chromatography-isotope ratio MS, stable isotopes, ureamia, urea dilution. Abbreviations: CV, coefficient of variation; GC-IRMS, gas chromatography...
View article titled, Determination of Urea Kinetics by Isotope Dilution with [ 13 C]Urea and Gas Chromatography–isotope Ratio Mass Spectrometry (GC–IRMS) Analysis
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Articles

Postprandial Protein Utilization and Protein Quality Assessment in Man

D. Joe Millward, Paul J. Pacy
Journal: Clinical Science
Clin Sci (Lond) (1995) 88 (6): 597–606.
https://doi.org/10.1042/cs0880597
Published: 01 June 1995
...D. Joe Millward; Paul J. Pacy © 1995 The Biochemical Society and the Medical Research Society 1995 diurnal cycling fasting feeding indispensable amino acid requirements leucine nitrogen balance protein turnover stable isotopes Clinical Science (1995) 88, 5 9 7 4 6 (Printed...
View article titled, Postprandial Protein Utilization and Protein Quality Assessment in Man
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Articles

Acute Stimulation of Albumin Synthesis Rate with Oral Meal Feeding in Healthy Subjects Measured with [ Ring - 2 H 5 ]Phenylalanine

K. A. Hunter, P. E. Ballmer, S. E. Anderson, J. Broom, P. J. Garlick, M. A. McNurlan
Journal: Clinical Science
Clin Sci (Lond) (1995) 88 (2): 235–242.
https://doi.org/10.1042/cs0880235
Published: 01 February 1995
...’ amount (43 mg/kg body weight) of phenylalanine enriched to 7.5, 10 or 15 atoms% with the stable isotope [ ring - 2 H 5 ]phenylalanine. 2. In one set of subjects, consumption of five small hourly meals resulted in a consistent and significant increase ( P < 0.05) in albumin fractional synthesis rate...
View article titled, Acute Stimulation of Albumin Synthesis Rate with Oral Meal Feeding in Healthy Subjects Measured with [ Ring - 2 H 5 ]Phenylalanine
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Articles

Increased Hepatic Secretion of Very-Low-Density Lipoprotein Apolipoprotein B-100 in Obesity: A Stable Isotope Study

Michael H. Cummings, Gerald F. Watts, Cauvery Pal, Margot Umpleby, Thomas R. Hennessy, Rossitza Naoumova, Peter H. Sönksen
Journal: Clinical Science
Clin Sci (Lond) (1995) 88 (2): 225–233.
https://doi.org/10.1042/cs0880225
Published: 01 February 1995
...Michael H. Cummings; Gerald F. Watts; Cauvery Pal; Margot Umpleby; Thomas R. Hennessy; Rossitza Naoumova; Peter H. Sönksen 1. We measured the hepatic secretion of very-low-density lipoprotein apolipoprotein B-100 (VLDL apoB) using a stable-isotope gas chromatography—mass spectrometry method in six...
View article titled, Increased Hepatic Secretion of Very-Low-Density Lipoprotein Apolipoprotein B-100 in Obesity: A <span class="search-highlight">Stable</span> <span class="search-highlight">Isotope</span> Study
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Articles

Recovery of 13 C in Breath from Infused NaH 13 CO 3 Increases During Euglycaemic Hyperinsulinaemia

D. Reaich, K. A. Graham, B. G. Cooper, C. M. Scrimgeour, T. H. J. Goodship
Journal: Clinical Science
Clin Sci (Lond) (1994) 87 (4): 415–419.
https://doi.org/10.1042/cs0870415
Published: 01 October 1994
... committee of Newcastle Health Authority and the University of Newcastle upon Type. Isotopes and glucose NaHI3CO3 (99 mol% 13C) was purchased from Promochem, St Albans, Herts, U.K. It was diluted ~~ Key words bicarbonate, carbon dioxide, hyperinsulinaemic clamp, indirect calorimetry, stable isotopes...
View article titled, Recovery of 13 C in Breath from Infused NaH 13 CO 3 Increases During Euglycaemic Hyperinsulinaemia
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Articles

Nitrogen Homoeostasis in Man: Diurnal Changes in Nitrogen Excretion, Leucine Oxidation and Whole Body Leucine Kinetics during a Reduction from a High to a Moderate Protein Intake

Marcelo R. Quevedo, Gill M. Price, David Halliday, Paul J. Pacy, D. Joe Millward
Journal: Clinical Science
Clin Sci (Lond) (1994) 86 (2): 185–193.
https://doi.org/10.1042/cs0860185
Published: 01 February 1994
... and the Medical Research Society 1994 adaptation diurnal cycling labile protein reserves nitrogen balance protein requirements stable isotopes Clinical Science (1994) 86, 185-193 (Printed in Great Britain) Nitrogen homoeostasis in man: diurnal changes in nitrogen excretion, leucine oxidation...
View article titled, Nitrogen Homoeostasis in Man: Diurnal Changes in Nitrogen Excretion, Leucine Oxidation and Whole Body Leucine Kinetics during a Reduction from a High to a Moderate Protein Intake
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Articles

Nitrogen Homoeostasis in man: Influence of Protein Intake on the Amplitude of Diurnal Cycling of Body Nitrogen

Gill M. Price, David Halliday, Paul J. Pacy, Marcelo R. Quevedo, D. Joe Millward
Journal: Clinical Science
Clin Sci (Lond) (1994) 86 (1): 91–102.
https://doi.org/10.1042/cs0860091
Published: 01 January 1994
... of daily losses and gains of both N and amino acids continues to increase up to very high protein intakes. Key words: diurnal cycling, leucine. nitrogen balance, phenyldanine. protein requirements. stable isotopes. Abbreviations: FFM, ht-free m u ; GP. generous protein; HP. high protein; KIC...
View article titled, Nitrogen Homoeostasis in man: Influence of Protein Intake on the Amplitude of Diurnal Cycling of Body Nitrogen
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Articles

Nitrogen Homoeostasis in man: The Diurnal Responses of Protein Synthesis and Degradation and Amino Acid Oxidation to Diets with Increasing Protein Intakes

Paul J. Pacy, Gill M. Price, David Halliday, Marcello R. Quevedo, D. Joe Millward
Journal: Clinical Science
Clin Sci (Lond) (1994) 86 (1): 103–118.
https://doi.org/10.1042/cs0860103
Published: 01 January 1994
... status. INTRODUCTION In studies designed to examine whole body pro- Key words diurnal cycling. feeding, fasting. ["Nlglycine, phenyldanine. protein turnover. stable isotopes. tyrasine. Abbreviations APE, atoms per cent aces; FT,. free tri-iodo thyronine; GP, generous protein; HP, high protein; IGF-I...
View article titled, Nitrogen Homoeostasis in man: The Diurnal Responses of Protein Synthesis and Degradation and Amino Acid Oxidation to Diets with Increasing Protein Intakes
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Articles

Utilization of Non-Protein Nitrogen in Whey-Dominant Formulae by Low-Birth Weight Infants

P. Darling, L. J. Wykes, R. Clarke, A. Papageorgiou, P. B. Pencharz
Journal: Clinical Science
Clin Sci (Lond) (1993) 84 (5): 543–548.
https://doi.org/10.1042/cs0840543
Published: 01 May 1993
... nitrogen. infant formulae low-birthweight infants nitrogen non-protein nitrogen stable isotopes urea whey protein Clinical Science (1993) 84, 543-548 (Printed in Great Britain) 543 Utilization of non-protein nitrogen in wheydominant formulae by low-birthweight infants P. DARLING, L. 1. WYKES, R...
View article titled, Utilization of Non-Protein Nitrogen in Whey-Dominant Formulae by Low-Birth Weight Infants
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Articles

Limits of adaptation to a diet low in protein in normal man: urea kinetics

M. Danielsen, A. A. Jackson
Journal: Clinical Science
Clin Sci (Lond) (1992) 83 (1): 103–108.
https://doi.org/10.1042/cs0830103
Published: 01 July 1992
... The Biochemical Society and the Medical Research Society 1992 colonic function nitrogen protein requirements stable isotopes Clinical Science (1992) 83, 103-108 (Printed in Great Britain) I03 Limits of adaptation to a diet low in protein in normal man: urea kinetics M. DANIELSEN and A. A. JACKSON...
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Articles

Adaptation to a diet low in protein: effect of complex carbohydrate upon urea kinetics in normal man

M. Langran, B. J. Moran, J. L. Murphy, A. A. Jackson
Journal: Clinical Science
Clin Sci (Lond) (1992) 82 (2): 191–198.
https://doi.org/10.1042/cs0820191
Published: 01 February 1992
.... bananas colonic function fibre nitrogen nitrogen balance non-starch polysaccharides protein requirements stable isotopes Clinical Science (1992) 82, 191-198 (Printed in Great Britain) Adaptation to a diet low in protein: effect of complex carbohydrate upon urea kinetics in normal man 191 M...
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Articles

Measurement of protein synthesis in human skeletal muscle: further investigation of the flooding technique

M. A. McNurlan, P. Essen, S. D. Heys, V. Buchan, P. J. Garlick, J. Wernerman
Journal: Clinical Science
Clin Sci (Lond) (1991) 81 (s25): 557–564.
https://doi.org/10.1042/cs0810557
Published: 01 October 1991
... synthesis stable isotopes KIC, α-ketoisocaproate ClinicalScience (1991) 81,557-564 557 Measurement of protein synthesis in human skeletal muscle: further investigation of the flooding technique M. A. McNURLAN', P. ESSEN2, S. D. HEYS3, V. BUCHAN', P. J. GARLICK' AND J. WERNERMAN* 'Rowett Research...
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Articles

Measurement of the Rate of Protein Synthesis in Muscle of Postabsorptive Young Men by Injection of a ‘Flooding Dose’ of [1- 13 C]leucine

Peter J. Garlick, Jan Wernerman, Margaret A. McNurlan, Pia Essen, Gerald E. Lobley, Eric Milne, Graham A. Calder, Eric Vinnars
Journal: Clinical Science
Clin Sci (Lond) (1989) 77 (3): 329–336.
https://doi.org/10.1042/cs0770329
Published: 01 September 1989
... volunteers and patients. 22 2 1989 6 3 1989 © 1989 The Biochemical Society and the Medical Research Society 1989 leucine mass spectrometry muscle protein synthesis stable isotopes Clinical Science (1989) 77,329-336 329 Measurement of the rate of protein synthesis in muscle...
View article titled, Measurement of the Rate of Protein Synthesis in Muscle of Postabsorptive Young Men by Injection of a ‘Flooding Dose’ of [1- 13 C]leucine
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Articles

Influence of amino acid administration on whole-body leucine kinetics and resting metabolic rate in postabsorptive normal subjects

P. J. Pacy, J. S. Garrow, G. C. Ford, H. Merritt, D. Halliday
Journal: Clinical Science
Clin Sci (Lond) (1988) 75 (3): 225–231.
https://doi.org/10.1042/cs0750225
Published: 01 September 1988
... The Biochemical Society and the Medical Research Society 1970 postabsorptive whole-body leucine kinetics resting metabolic rate stable isotopes steady-state reciprocal pool model Clinical Science (1988) 75, 225-231 225 Influence of amino acid administration on whole-body leucine kinetics and resting...
View article titled, Influence of amino acid administration on whole-body leucine kinetics and resting metabolic rate in postabsorptive normal subjects
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Articles

Rate of protein synthesis in skeletal muscle of normal man and patients with muscular dystrophy: A reassessment

D. Halliday, P. J. Pacy, K. N. Cheng, F. Dworzak, J. N. A. Gibson, M. J. Rennie
Journal: Clinical Science
Clin Sci (Lond) (1988) 74 (3): 237–240.
https://doi.org/10.1042/cs0740237
Published: 01 March 1988
... synthetic rate. 7 7 1987 24 8 1987 © 1970 The Biochemical Society and the Medical Research Society 1970 muscle protein synthesis stable isotopes Clinical Science ( 1 988) 74,237-240 237 Rate of protein synthesis in skeletal muscle of normal man and patients with muscular dystrophy...
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Articles

Influence of fasting on leucine and muscle protein metabolism across the human forearm determined using l-[1-13C,15N]leucine as the tracer

K. N. Cheng, P. J. Pacy, F. Dworzak, G. C. Ford, D. Halliday
Journal: Clinical Science
Clin Sci (Lond) (1987) 73 (3): 241–246.
https://doi.org/10.1042/cs0730241
Published: 01 September 1987
... in future studies on muscle protein metabolism. 21 11 1986 3 3 1987 © 1987 The Biochemical Society and the Medical Research Society 1987 forearm leucine metabolism protein metabolism stable isotopes Clinical Science (1987) 73, 241-246 Influence of fasting on leucine and muscle...
View article titled, Influence of fasting on leucine and muscle protein metabolism across the human forearm determined using l-[1-13C,15N]leucine as the tracer
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Articles

Assessment of Glucose Turnover in Normal Man with the Use of a Non-Radioactive Isotopically Labelled Preparation, [6,6- 2 H]Glucose, as Tracer

J. W. Haigh, D. G. Johnston, A. J. McCulloch, M. F. Laker, J. Welby, S. Evans
Journal: Clinical Science
Clin Sci (Lond) (1982) 63 (5): 437–440.
https://doi.org/10.1042/cs0630437
Published: 01 November 1982
... Research Society 1982 glucose kinetics glucose metabolism glucose turnover mass spectrometry stable isotopes tracer methodology Clinical Science (1982) 63,431440 437 Assessment of glucose turnover in normal man with the use of a non-radioactive isotopically labelled preparation, [6,6-2H...
View article titled, Assessment of Glucose Turnover in Normal Man with the Use of a Non-Radioactive Isotopically Labelled Preparation, [6,6- 2 H]Glucose, as Tracer
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