... of serpina3c in atherosclerosis after HFD for 12 weeks. Compared with Apoe −/− mice, the Apoe −/− /serpina3c −/− mice developed more severe atherosclerosis, and the number of VSMCs and macrophages in aortic plaques was significantly increased. The present study revealed serpina3c as a novel thrombin inhibitor...

... the manner in which normoxic and hypoxic exercise regimens affect procoagulant monocyte-derived microparticle (MDMP) formation and monocyte-promoted thrombin generation (TG). Forty sedentary healthy males were randomized to perform either normoxic (NET; 21% O 2 , n =20) or hypoxic (HET; 15% O 2 , n =20...

...Shin-Ei Cheng; I-Ta Lee; Chih-Chung Lin; Li-Der Hsiao; Chuen-Mao Yang Up-regulation of ICAM-1 (intercellular adhesion molecule-1) is frequently implicated in lung inflammation and lung diseases, such as IPF (idiopathic pulmonary fibrosis). Thrombin has been shown to play a key role in inflammation...

..., both in the absence and presence of extracellular Ca 2+ . Platelet stimulation with thrombin in the absence and presence of extracellular Ca 2+ induced a rapid rise in [Ca 2+ ] i that was of greater magnitude in platelets from BDL rats than in controls. Ca 2+ storage was significantly elevated...

...-to-moderate essential hypertension. Fibrinogen, thrombin–antithrombin complex (TAT) and Factor VII were determined in plasma at rest and after a mental stress test following placebo for 6 weeks, or ramipril for 6 weeks or 6 months. Ramipril reduced resting TAT, and tended to reduce fibrinogen; Factor VII...

.... A secondary objective was to assess the relationship between the structures of the proteins and their inhibitory activities. Prothrombin was purified from Prothrombinex-HT, cleaved with thrombin and the resulting fragment 1 (F1) and fragment 2 (F2) were purified. The purity of each protein was confirmed...

...N. Håkan WALLÉN; Alison H. GOODALL; Nailin LI; Paul HJEMDAHL Stress-induced activation of haemostasis may be involved in the triggering of acute coronary syndromes. We compared the effects of mental stress, dynamic exercise and adrenaline infusion on platelet sensitivity to thrombin using flow...

...Marcus K. Ilton; Marcia L. Taylor; Neil L. A. Misso; Philip J. Thompson; Joseph Hung 1. Close contact between platelets and neutrophils modulates their cellular interactions in thrombotic and inflammatory states, with stimulation of P-selectin expression on platelets by agonists such as thrombin...

... the efflux of 86 Rb + (a marker for K + ) from 86 Rb + -loaded cells, and have defined their responses to stimulation by the platelet agonist thrombin and the calcium ionophore ionomycin. 3. Thrombin (0.1–0.6 i.u./ml) stimulated an increase in 86 Rb + efflux from the platelets in a concentration-dependent...

...R. M. Touyz; E. L. Schiffrin 1. Endothelin-1 has anti-aggregatory properties, but the mechanism underlying this inhibitory action is unknown. This in vitro study investigates effects of endothelin-1 on thrombin-stimulated aggregation and intracellular free calcium concentration in human platelets...

... feature of septicaemia. We hypothesized that thrombin generated during disseminated intravascular coagulation might modulate the changes in vascular tone induced by endotoxin. 2. Incubation of rat aortic rings for 4 h with α-thrombin (0.003–3.0 NIH units/ml) did not change their reactivity...

... or thrombin was unaffected by aspirin. 4. Under the conditions of this assay, platelets undergo a partial degranulation of α-granules and lysosomes (evidenced by expression of P-selectin and CD63, respectively) in response to ADP, and full degranulation in response to thrombin. P-selectin expression...

... h of incubation with added thrombin (stimulated production) or media (basal production). Supernatants were then collected for measurement of 6-keto-prostaglandin F 1α by radioimmunoassay. 3. Basal production of 6-keto-prostaglandin F 1α was greater in response to serum from non-pregnant women than...

.... These results suggest that structural membrane modifications may participate in the various functional abnormalities observed in platelets from hypertensive patients. Key words: cytosolic Ca2+, essential hypertension, fluor- escence depolarization, membrane fluidity, platelets, thrombin. Abbreviations: DPH, 1,6...

...C. C. T. Smith; A. P. Wilson; B. N. C. Prichard; D. J. Betteridge 1. Using high-performance liquid chromatography with electrochemical detection, we have studied the release of endogenous catecholamines from washed platelets induced to aggregate by ADP and thrombin. 2. Washed platelets exhibit...