... Society © 2001 2001 111Clinical Science (2001) 100, 111 116 (Printed in Great Britain) Homocysteine and thiol metabolites in vitamin B12 deficiency L. R. RANGANATH*, M. BAINES and N. B. ROBERTS *Department of Chemical Pathology, Epsom General Hospital, Epsom, Surrey KT18 7EG, U.K., and Department...

...) 47 I Demonstration of hypomethylation of proteins in the brain of pigs (but not in rats) associated with chronic vitamin B12 inactivation M. McKEEVER, A. MOLLOY*, P. YOUNG?, S. KENNEDY?, D. G. KENNEDY?, 1. M. SCOTT and D. G. WEIR* Departments of Biochemistry and *Clinical Medicine, Trinity College...

... I1 and to subsequent increased binding of [57Co]Cbl t o the enzyme proteins (Fig. 4). Yagiri [41] also observed a higher rate of conversion of [57Co]CNCbl to aden~sylCo]cobalarnin i vitamin B12-deficient rat liver. The presence of a large pool of free cobalamin in the kidney indicates a unique role...

.... After fractionation of the vitamin B12- binding proteins of ten normal sera the com- ponents containing transcobalamin I1 and R-type binders of vitamin B12 respectively were studied for endogenous vitamin B12 content by two distinct systems of vitamin BIZ assay. 2. The measurements of total serum...

... containing trans- cobalamin I and transcobalamin 111. Cyano- cobalamin was found in both fractions in two cases, in the transcobalamin 111 fraction only in one case and was absent in one case. Key words: transcobalamins, vitamin B1 2. Abbreviations : TC, transcobalamin. Introduction Four forms of vitamin B12...

.... Serum from normal subjects has been chromato- graphed on Sephadex G-200 columns and the fractions containing transcobalamins 0, I and I1 have been identified. 2. The fractions corresponding to transcobalamin I contained, on average, 90% of the endogenous vitamin B12. Only 3% was attached to trans...

...W. G. E. Cooksley; A. S. Tavill 1. The rate of radioactive vitamin B12 excretion into plasma and bile from the isolated perfused rat liver and into bile in vivo has been measured after the intramuscular injection of radioactive cyanocobalamin at various time-intervals before study. 2...

... granulocytic leukaemia (experiment a). Details are as given in Fig. 1. The percentages of recovered activity for components are : alkaline phosphatase (4-methylumbellifery1 phosphate), 95; alkaline phosphatase (p-nitrophenyl phos- phate), 104; malate dehydrogenase, 82; vitamin B12, 103; 5'-nucleotidase, 77...

... (Table 1). The patients comprised four males and six females. Their haemoglobin concentrations ranged from 5.1 to 10.5 g/dl and all showed florid megaloblastic marrow appearances with adequate iron stores, and malabsorption of vitamin B,, corrected by intrinsic factor. The serum vitamin B12 con...

... into plasma or bile. 9. It is concluded that the fluxes of hepatic vitamin B12 and TCII are very rapid and that the release of vitamin B12 by the rat liver is controlled in the short term by Correspondence: Dr A. S. Tavill, Clinical Research Centre, Watford Road, Harrow HA1 3UK, Middlesex. 53 1 532 W. G. E...

... plasma vitamin B12 appeared to be slightly higher in patients than controls but the difference was not significant at the 5% level (0.1 >P>0.05). Concentrations of Me-B,, and OH-B,, did not differ significantly between groups but the mean plasma Ado-B,, was almost twice as high in the patients...

... 171 ADAMS, J.F. (1962) The measurement of the total assayable vitamin B12 in the body. In: Vitamin B12 and Intrinsic Factor. 2nd Europaisches Symposion, Hamburg, 1961, p. 397. Ed. by Heinrich, H.C. Enke, Stutt- gart. ANDERSON, B.B. (1965) Investigations into the Euglena method of assay of vitamin BIZ...

... in the same position as transcobalamin I1 on column chromatography. 3. It was concluded that transcobalamin I1 is synthesized by rat liver. Key words : vitamin B12, transcobalamin, liver. Normal serum contains two vitamin Bbinding proteins, transcobalamin I (TC I) and I1 (TC 11). TC I (molecular weight 120000...