Micro-electron diffraction, or MicroED, is a structure determination method that uses a cryo-transmission electron microscope to collect electron diffraction data from nanocrystals. This technique has been successfully used to determine the high-resolution structures of many targets from crystals orders of magnitude smaller than what is needed for X-ray diffraction experiments. In this review, we will describe the MicroED method and recent structures that have been determined. Additionally, applications of electron diffraction to the fields of small molecule crystallography and materials science will be discussed.
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Review Article| February 06 2018
MicroED: a versatile cryoEM method for structure determination
Brent L. Nannenga ;
2Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, U.S.A.
3Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, U.S.A.
4Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, U.S.A.
Correspondence: Tamir Gonen (firstname.lastname@example.org)
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Marcellus Ubbink, Anastassis Perrakis, Brent L. Nannenga, Tamir Gonen; MicroED: a versatile cryoEM method for structure determination. Emerg Top Life Sci 20 April 2018; 2 (1): 1–8. doi: https://doi.org/10.1042/ETLS20170082
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