In recent years, it has become evident that structural characterization would gain significantly in terms of biological relevance if framed within a cellular context, while still maintaining the atomic resolution. Therefore, major efforts have been devoted to developing Cellular Structural Biology approaches. In this respect, in-cell NMR can provide and has provided relevant contributions to the field, not only to investigate the structural and dynamical properties of macromolecules in solution but, even more relevant, to understand functional processes directly in living cells and the factors that modulate them, such as exogenous molecules, partner proteins, and oxidative stress. In this commentary, we review and discuss some of the main contributions to the understanding of protein structural and functional properties achieved by in-cell NMR.
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April 2018
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Cover Image
Cryo-STX projection image showing a parasitophorous vacuole (yellow) within a human fibroblast cell, containing four Toxoplasma gondii parasites (membranes in cyan, nuclei in red and rhoptries in green). In this issue of Emerging Topics in Life Sciences, Harkiolaki et al. describe the use of Cryo-soft X-ray tomography to explore the ultrastructure of whole cells. Image kindly provided by Professor Helen Saibil (Birkbeck College, London, U.K.). For further details, see pages 81–92.
Review Article|
February 06 2018
New structural and functional insights from in-cell NMR
Enrico Luchinat
;
Enrico Luchinat
1Magnetic Resonance Center — CERM, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019 Florence, Italy
2Department of Experimental and Clinical Biomedical Sciences ‘Mario Serio’, University of Florence, Viale Morgagni 50, 50134 Florence, Italy
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Lucia Banci
1Magnetic Resonance Center — CERM, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019 Florence, Italy
3Department of Chemistry, University of Florence, Via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy
Correspondence: Lucia Banci (banci@cerm.unifi.it)
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Emerg Top Life Sci (2018) 2 (1): 29–38.
Article history
Received:
November 28 2017
Revision Received:
December 13 2017
Accepted:
December 18 2017
Citation
Marcellus Ubbink, Anastassis Perrakis, Enrico Luchinat, Lucia Banci; New structural and functional insights from in-cell NMR. Emerg Top Life Sci 20 April 2018; 2 (1): 29–38. doi: https://doi.org/10.1042/ETLS20170136
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