Microbial cell factories are extensively used for the biosynthesis of value-added chemicals, biopharmaceuticals, and biofuels. Microbial biosynthesis is also realistic for the production of heterologous molecules including complex natural products of plant and microbial origin. Glycosylation is a well-known post-modification method to engineer sugar-functionalized natural products. It is of particular interest to chemical biologists to increase chemical diversity of molecules. Employing the state-of-the-art systems and synthetic biology tools, a range of small to complex glycosylated natural products have been produced from microbes using a simple and sustainable fermentation approach. In this context, this review covers recent notable metabolic engineering approaches used for the biosynthesis of glycosylated plant and microbial polyketides in different microorganisms. This review article is broadly divided into two major parts. The first part is focused on the biosynthesis of glycosylated plant polyketides in prokaryotes and yeast cells, while the second part is focused on the generation of glycosylated microbial polyketides in actinomycetes.
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Cover Image
Cover Image
The lack of availability of glycans and glycoconjugates has severely limited the development of the field of glycobiology. This issue of Emerging Topics in Life Sciences features nine reviews by respected scientists in the field and deals with the application of metabolic engineering to the production of these complex carbohydrate or saccharide structures. The cover image illustrates that capsular polysaccharides and other bioengineered compounds can be generated by engineered cells, such as Escherichia coli, through the application of various metabolic engineering tools and strategies. The image was kindly provided by Robert J. Linhardt (Rensselaer Polytechnic Institute) and designed by Asher Williams (Rensselaer Polytechnic Institute).
Metabolic engineering of glycosylated polyketide biosynthesis Available to Purchase
Mattheos A.G. Koffas, Robert J. Linhardt, Ramesh Prasad Pandey, Prakash Parajuli, Jae Kyung Sohng; Metabolic engineering of glycosylated polyketide biosynthesis. Emerg Top Life Sci 26 October 2018; 2 (3): 389–403. doi: https://doi.org/10.1042/ETLS20180011
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