Biosensors that depend on a physical or chemical measurement can be adversely affected by non-specific interactions. For example, a biosensor designed to measure specifically the levels of a rare analyte can give false positive results if there is even a small amount of interaction with a highly abundant but irrelevant molecule. To overcome this limitation, the biosensor community has frequently turned to antibody molecules as recognition elements because they are renowned for their exquisite specificity. Unfortunately antibodies can often fail when immobilised on inorganic surfaces, and alternative biological recognition elements are needed. This article reviews the available non-antibody-binding proteins that have been successfully used in electrical and micro-mechanical biosensor platforms.
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June 2016
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Schematic representation of the Affimer® protein. Affimer® molecules are small single domain proteins, engineered to be an alternative to antibodies. Image kindly supplied by Paul Ko Ferrigno. For more information see pages 19-25.Close Modal - PDF Icon PDF LinkTable of Contents
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Review Article|
June 30 2016
Non-antibody protein-based biosensors
Paul Ko Ferrigno
1Avacta Life Sciences Ltd., Unit 651A, Street 5, Thorp Arch Estate, Wetherby LS23 7FZ, U.K.
Correspondence: Paul Ko Ferrigno (paul.koferrigno@avacta.com)
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Essays Biochem (2016) 60 (1): 19–25.
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Pedro Estrela, Paul Ko Ferrigno; Non-antibody protein-based biosensors. Essays Biochem 30 June 2016; 60 (1): 19–25. doi: https://doi.org/10.1042/EBC20150003
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