Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence correlation spectroscopy) on an optical (confocal) microscope is a popular tool for investigating such dynamics. Unfortunately, its full applicability is constrained by the limited spatial resolution of a conventional optical microscope. The present chapter depicts the combination of optical super-resolution STED (stimulated emission depletion) microscopy with FCS, and why it is an important tool for investigating molecular membrane dynamics in living cells. Compared with conventional FCS, the STED-FCS approach demonstrates an improved possibility to distinguish free from anomalous molecular diffusion, and thus to give new insights into lipid–protein interactions and the traditional lipid ‘raft’ theory.
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Review Article| February 06 2015
Super-resolution optical microscopy of lipid plasma membrane dynamics
Christian Eggeling 1
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, U.K.
1To whom correspondence should be addressed (email email@example.com).
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Essays Biochem (2015) 57: 69–80.
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Ingela Parmryd, Christian Eggeling; Super-resolution optical microscopy of lipid plasma membrane dynamics. Essays Biochem 15 February 2015; 57 69–80. doi: https://doi.org/10.1042/bse0570069
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