Membrane lipids form the basic framework of biological membranes by forming the lipid bilayer, but it is becoming increasingly clear that individual lipid species play different functional roles. However, in comparison with proteins, relatively little is known about how lipids are distributed in the membrane. Several microscopic methods are available to study membrane lipid dynamics in living cells, but defining the distribution of lipids at the submicrometre scale is difficult, because lipids diffuse quickly in the membrane and most lipids do not react with aldehydes that are commonly used as fixatives. Quick-freezing appears to be the only practical method by which to stop the lipid movement instantaneously and capture the molecular localization at the moment of interest. Electron microscopic methods, using cryosections, resin sections, and freeze-fracture replicas are used to visualize lipids in quick-frozen samples. The method that employs the freeze-fracture replica is unique in that it requires no chemical treatment and provides a two-dimensional view of the membrane.
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Review Article| February 06 2015
Microscopy of membrane lipids: how precisely can we define their distribution?
Sho Takatori ;
Essays Biochem (2015) 57: 81–91.
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Ingela Parmryd, Sho Takatori, Toyoshi Fujimoto; Microscopy of membrane lipids: how precisely can we define their distribution?. Essays Biochem 15 February 2015; 57 81–91. doi: https://doi.org/10.1042/bse0570081
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