The many length-scales of DNA packaging: The building block of chromatin is the nucleosome (blue discs), which is folded and twisted to higher levels of genome organisation, up to mitotic chromosomes (red). Mixed media artwork by Shelagh Boyle
Single cell biology is a new field combining multiple disciplines and using newly developed techniques to explore and understand life at the level of a single cell. This issue contains reviews on single cell imaging, single-cell RNA sequencing, controlling protein functions in cells and CRISPR-based immune defence.
Mosaic panels show cells (indicated by nuclei in blue) surrounded by immunofluorescently stained extracellular matrix fibrils and are over-laid with (from the top), diagram of cross-linked collagen molecules (see article by Piersma and Bank), schematic of laminin molecule (see article by Hohenester) and model of tissue transglutaminase active site (see article by Draper et al.) to exemplify the complexity of protein interactions that build the extracellular matrix. Read more about the extracellular matrix in the latest issue of Essays in Biochemistry (volume 63, issue 3).